Slide Brite

SOLUTION:

Additionally Needed:

For storage requirements and expiration date refer to individual bottle labels.

APPLICATION:

Newcomer Supply Slide Brite™ Xylene Substitute is classified as an aliphatic hydrocarbon that provides a safe alternative to xylene, reduces risks and improves personnel safety in the laboratory. Benefits of Slide Brite™ include:

• • • Odorless, non-hazardous, non-irritating and fast drying.
    • Gentle on tissue and enhanced nuclear detail.
    • No tissue brittleness, shrinkage or adverse morphologic changes.
    • Compatible with IHC staining.
    • Does not require hazardous/flammable storage.
    • Flash point of 61°C/142°F (29°C/84°F flash point of xylene)
    • No vapor monitoring.
    • Compatible on tissue processors and staining systems.

METHOD:

Fixation: Formalin 10%, Phosphate Buffered (Part 1090)
Technique:  Paraffin, frozen sections, smears

PROCESSING PROCEDURE: 

1. 1. Refer to Slide Brite™ Tissue Processing Schedule below.
   2. Use three Slide Brite™ clearing stations, 60 minutes each.
      1. 1. For two clearing stations, allow 90 minutes per station.
   3. Rotate, filter and/or replace Slide Brite™ stations daily or after processing approximately 1000 blocks.
   4. Test and optimize Slide Brite™ as a clearing agent in tissue processing schedules prior to standard use.

STAINING PROCEDURE:

1. 1. Refer to Slide Brite™ Staining Procedure below.
   2. For best deparaffinization results, place warm slides directly from dryer/oven into Slide Brite™.
   3. Deparaffinize warm slides thoroughly in three changes of Slide Brite™, 3 minutes each. Hydrate through two changes each of 100% and 95% ethyl alcohols, 10 dips each.  Wash well with distilled water.
      1. 1. See Procedure Notes #1, #2 and #3.
   4. Proceed with staining protocol.
   5. Dehydrate in two changes of 95% and three changes of 100% ethyl alcohol. Clear in four changes of Slide Brite™.
   6. Coverslip with Choice Mounting Medium (Part 1032).
      1. 1. See Procedure Note #4.
   7. Test and optimize Slide Brite™ in staining procedures and automated staining systems prior to standard use.

PROCEDURE NOTES:

1. 1. Deparaffinization and clearing steps may require longer timings then xylene.
   2. Slide Brite™ requires more frequent changes compared to xylene.
   3. Any water contamination will layer on top of Slide Brite™.
      1. 1. Use Hydrosorb-X™ Water Absorbing Packets (Part STHXP) to minimize presence of water.
   4. Test Slide Brite™ compatibility with other mounting mediums prior to use.
      1. 1. If mounting medium displays separation or is not readily miscible, it is incompatible with Slide Brite™.
   5. Slide Brite™ is not recommended for automated coverslippers.
   6. Slide Brite™ will not remove adhered coverslips as well as xylene.
   7. Refer to manufacturer’s specifications on the use of Slide Brite™ on all instrumentation.

REFERENCES:

1. 1. Dapson, Janet Crookham, and Richard W. Dapson. Hazardous Materials in the Histopathology Laboratory: Regulations, Risks, Handling and Disposal. 4^th Battle Creek, MI: Anatech, 2005. 150-155, 235.
   2. Wynnchuk, Maria. “Evaluation of Xylene Substitutes for Paraffin Tissue Processing.” The Journal of Histotechnology2 (1994): 143-149.
   3. Modifications developed by Newcomer Supply Laboratory.

Slide Brite™ Routine Tissue Processing Schedule

Slide Brite™ Routine Tissue Processing Notes:

1. 1. When using only two clearing stations, increase time from 60 minutes to 90 minutes per station (Steps #8 to #10).
   2. Rotate, filter and/or replace Slide Brite™ solutions daily or after processing approximately 1000 blocks.

Slide Brite™ Staining Procedure                                                                                               

Slide Brite™ Staining Procedure Notes:

1. 1. For best deparaffinization results, place warm slides directly from dryer/oven into Slide Brite™ (Step #2).
   2. Choice Mounting Medium (Part 1032) is the recommended mounting medium with Slide Brite™.