REAGENTS, CHEMICALS & BUFFERS

ACETIC ACID Glacial,ACS

ACETIC ACID 0.5% Aqueous Solution
(use: Trichrome, Gomori One-Step.)

ACETIC ACID 1% Aqueous Solution
(use: Masson & Gomori Trichrome & May-Grunwald Giemsa.)

ACETIC ACID 3% Aqueous Solution
(use: Alcian Blue, pH 2.5.)

ACETONE ACS
(use: Various, including Brown-Brenn & Hucker-Twort Gram Stains.)

 
 
 
 
 
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ACETONE-ALCOHOL 1:1
(use: Gram Stain Brown-Brenn)

ACETONE/XYLENE 1:1
(use: Brown-Brenn & Brown-Hopps Gram Stains.)

ACID-ALCOHOL 1% Solution
(use: H&E, AFB, Brown-Brenn, PAS & Fite Stains.)

ACIDULATED WATER pH 4.0-4.1
(use: Warthin-Starry Method.)

ALCOHOL DENATURED ACS
(FYI: No ketone and less Isopropyl.)
See also Ethyl Alcohol Denatured.

AMMONIUM HYDROXIDE 28-30%,ACS
(use: Reticulum, Gordon & Sweet; Lester King, Bielchowsky.)

BORAX (Sodium Borate) 5% Saturated Alcoholic Solution
(use: Sulfated Alcian Blue for Amyloid.)

BUFFERS
PH applies at 25°C. (Shelf Life 6 mos. Store @ 4°C.) No preservatives. Mercury free.

CITRATE BUFFER 0.01M, pH 6.0
(use: Epitope retrieval)

EDTA BUFFER 0.001M, pH 8.0
(use: Epitope retrieval)

HYALURONIDASE BUFFER
(use: Buffer for digestion in Alcian Blue pH 2.5. Does not include enzyme.)

MILLONIG BUFFER, pH 7.0
(use: To make Millonig Glutaraldehyde Fixative for EM.)

PHOSPHATE BUFFER, pH 7.0
(use: With Giemsa Stain.)

PHOSPHATE BUFFER, pH 6.0
(use: Buffer for Glycogen Digestion; Does not include enzyme.)

PHOSPHATE BUFFER 0.1M, pH 7.2-7.4
(use: IHC wash and rinse.)

PHOSPHATE BUFFERED SALINE 0.01M, pH 7.2
(use: IHC diluent, rinse and wash.)

PHOSPHATE BUFFERED SALINE 0.01M, pH 7.4
(use: IHC diluent, rinse and wash.)

PHOSPHATE BUFFERED SALINE 0.05M, pH 7.4
(use: IHC wash solution.)

PHOSPHATE BUFFERED SALINE 0.1M, pH 7.4
(use: Rinsing unfixed tissues and diluting antibodies, eg. in IHC.)

TRIS BUFFER 0.05M, pH 7.6
(use: Working solution for IHC diluent, rinse and wash.)

TRIS BUFFERED SALINE 0.05M, pH 7.6, 10X
(use: IHC wash solution; dilute 1:10 before use.)

TRIS BUFFERED SALINES used with FrozFix®
See FROZFIX in Fixatives Section.

WRIGHT STAIN BUFFER SOLUTION, pH 6.8

CHROMIC ACID 10% Aqueous Solution
(use: Stock solution for Grocott Solution GMS.)

CHROMIC ACID 5% Aqueous Solution
(use: Working dilution for Grocott Solution GMS.)

COLLOIDAL IRON Stock Solution
(use: Mowry Colloidal Iron.)

DECALCIFICATION END-POINT Set
Sol'n A: Ammonium Hydroxide, 5% Aqueous Solution
Sol'n B: Ammonium Oxalate, 5% Aqueous Solution
(use: Determination of complete decalcification of specimen.)

DECALCIFYING SOLUTION EDTA/Sucrose
(use: For large bones.)
Gentle enough for IHC & IF.

DECALCIFYING SOLUTION Formic/Citrate
Standard gentle formula.

 
 
Freight Hauler Only

DECALCIFYING SOLUTION Formic Acid, 5% Aqueous Solution

 
Freight Hauler Only
Freight Hauler Only

DECALCIFYING SOLUTION Dilute HCl with EDTA
(use: Excellent for bone marrows. Gentle but quick!)

ETHYL ALCOHOL, DENATURED
(FYI: Higher % Isopropyl Alcohol & contains ketone.)
See also Alcohol Denatured ACS.

100%
 
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95%
 
Ship Ground Only

80%
 
Ship Ground Only

70%
 
Ship Ground Only

FERRIC CHLORIDE 1% Aqueous Solution
(use: Schmorl Melanin Stain.)

FERRIC CHLORIDE 2% Aqueous Stock Solution
(use: Working solution for VVG Elastic Stain.)

FERRIC CHLORIDE 10% Aqueous Solution
(use: Verhoeff Elastic & Hall Bile Stains.)

FERRIC CHLORIDE 29% Aqueous Stock Solution
(use: Weigert, Lillie & Mallory Iron Hematoxylins: Elastic and Schmorl Melanin stains. Can be diluted to 5% & 2%.)

FORMIC ACID 95%, ACS
(use: Additional step after fixation to reduce C-J infectivity.)

 
 
Freight Hauler Only

FROZFIX® CONDITIONING SET
See FROZFIX in Fixatives Section.

GALLEGO SOLUTION
(use: Brown-Hopps modified Gram stain.)

GOLD CHLORIDE 0.1% Aqueous Solution
(use: GMS Stain.).

GOLD CHLORIDE 0.2% Aqueous Solution
(use: Gordon & Sweets Reticulum, Fontana-Masson Argentaffin, and GMS Stains.)

GOLD CHLORIDE 0.25% Aqueous Solution
(use: Gomori Basement Membrane Stain.)

GOLD CHLORIDE 1% Aqueous Stock Solution
(use: Gordon & Sweets and Gomori Basement Membrane, Fontana-Masson Argentaffin, and GMS Stains. Can be diluted to 0.25%, 0.2% & 0.1%.)

HYALURONIDASE BUFFER
(use: Buffer for digestion in Alcian Blue pH 2.5. Does not include enzyme.)

HYDROCHLORIC ACID 0.1N Aqueous Solution
(use: Alcian Blue 1.0 pH Sulfated Mucosubstances)

HYDROCHLORIC ACID 36-38%,ACS

HYDROCHLORIC ACID 5% Aqueous Solution
(use: For acid cleaning glassware prior to silver staining.)

HYDROCHLORIC ACID 20% Aqueous Solution
(use: Gomori Mod. Iron Stain, Prussian Blue, & stock for Colloidal Iron.)

HYDROGEN PEROXIDE 30% Aqueous Solution
(Vented cap allows for release of pressure. Condensation inside shipping bag may occur.)

Ship Ground Only

IODINE SOLUTION Gram
(use: Brown-Brenn & Brown-Hopps Gram Stains and mercury pigment removal.)

IODINE SOLUTION Verhoeff
(use: Verhoeff Elastic/Masson Trichrome procedure.)

IODINE SOLUTION Weigert & Lugol
(use: Hucker-Twort Gram Stain,Verhoeff Elastic, McLetchie Trichrome and mercury pigment removal.)

ISOPROPYL ALCOHOL ACS

LITHIUM CARBONATE Saturated Aqueous Solution

METHENAMINE 3% Aqueous Solution
(use: Gomori Basement Membrane Stain (PAMM), & Urates.)

METHANOL Anhydrous,ACS
(use: Fixative for blood smears.)

MICHEL WASH SOLUTION
(Shelf Life 6 mos. Store @ 4°C.)

MOLD RELEASE Concentrate
(use: Help blocks release from embedding molds.)

OXALIC ACID 5% Aqueous Solution
(use: Bleach out melanin or phosphotungstic acid in the PTAH Stain, & is stock for Gordon & Sweets Reticulum.)

PERIODIC ACID 0.5% Aqueous Solution
(use: PAS for glycogen or amoeba, and in Gomori Basement Membrane Stain.)

PERIODIC ACID 1% Aqueous Solution
(use: Can be diluted to 0.5% for PAS or PAMM.)

PHOSPHATE BUFFER
See BUFFERS in Reagents, Chemicals & Buffers Section above.

PHOSPHOMOLYBDIC ACID 1% Aqueous Solution
(use: Klatskin Trichrome for liver.)

PHOSPHOMOLYBDIC ACID 10% Aqueous Solution

PHOSPHOMOLYBDIC - PHOSPHOTUNGSTIC ACID SOLUTION
(use: Masson Trichrome Stain.)

PHOSPHOTUNGSTIC - ACETIC ACID SOLUTION
(use: with Gomori One-Step Trichrome for Decolorization if needed.)

PHOSPHOTUNGSTIC ACID 2% Alcoholic Solution
(use: Trichrome, McLetchie)

PHOSPHOTUNGSTIC ACID 4% Alcoholic Solution

PHOSPHOTUNGSTIC ACID 5% Aqueous Solution
(use: Trichrome, Masson Light Green)

PICRIC ACID - ACETONE 0.1% Solution
(use: Gram Stains, eg. Brown Brenn.)

PICRIC ACID - ACETONE 0.05% Solution
(use: Brown & Hopps.)

PICRIC ACID Saturated Aqueous Stock Solution
(use: Instead of keeping the hazardous powder to make-up Bouin, etc.)

PICRIC ACID 7% Alcoholic Stock Solution
(use:Alternative to Bouin as mordant for the liver Trichrome, and as differentiator for Sulfated Alcian Blue for Myocardial Amyloid.)

POTASSIUM FERRICYANIDE 1% Aqueous Solution
(use: Schmorl Melanin Stain.)

POTASSIUM FERRICYANIDE 10% Aqueous Stock Solution
(use: Schmorl Method for reducing substances, eg. Melanin.)

POTASSIUM FERROCYANIDE 10% Aqueous Stock Solution
(use: Gomori Mod. Iron Stain & stock for Colloidal Iron.)

POTASSIUM PERMANGANATE 0.25% Aqueous Solution
(use: PTAH Stain & working solution for melanin bleaching.)

POTASSIUM PERMANGANATE 1% Aqueous Solution
(use: Gordon & Sweets Reticulum Stain & stock solution for melanin bleaching.)

PROPYLENE GLYCOL 100% ACS
(use: Oil Red O in Propylene Glycol, Fat Stain.)

PROPYLENE GLYCOL 0.85
(use: Oil Red O in Propylene Glycol, Fat Stain.)

ROSIN 10% Alcoholic Stock Solution
(use: Wolbach Giemsa.)

SCOTT TAP WATER Substitute

SLIDE BRITE™
An extremely safe, odorless alternative to xylene. Gentle on tissue and does not make it brittle. Excellent for derms and biopsy tissue. No tissue shrinkage or morphology changes. Enhances nuclear detail. Non-flammable with a flash point of 142°F. Use with S-Mount Mounting Medium for optimal results. No hazardous shipping fees! See Slide Brite in Safety Section.

SODIUM BICARBONATE 5% Aqueous Solution
(use: Original Brown & Brenn Gram Stain, and stock solution for Wolbach Giemsa.)

SODIUM BISULFITE 1% Aqueous Solution
(use: Grocott Methenamine Silver GMS Stain.)

SODIUM BORATE 0.5% Aqueous Solution
(use: Rhodanine Stain for Copper.)

SODIUM BORATE 5% Aqueous Solution
(use: Standard Grocott Methenamine Silver, GMS procedure.)

SODIUM BORATE 5% Alcoholic
See BORAX, cata #1019 in Reagents, Chemicals & Buffers Section.

SODIUM METABISULFITE 1% Aqueous Solution
(use:Working solution for GMS.)

SODIUM SULFATE 1% Aqueous Solution
(use: Sulfated Alcian Blue procedure for Amyloid stain.)

SODIUM THIOSULFATE 2% Aqueous Solution
(use: Working solution for GMS.)

SODIUM THIOSULFATE 2.5% Aqueous Solution
(use: Working solution for Gomori Basement Membrane Stain.)

SODIUM THIOSULFATE 3% Aqueous Solution
(use: Gomori Methenamine-Silver for Nitrates.)

SODIUM THIOSULFATE 5% Aqueous Solution
(use: Stock solution for Gomori Basement Membrane & GMS; Working solution for VVG Elastic, Gordon & Sweets and Mercury pigment removal.)

SULFURIC ACID 1% Aqueous Solution
(use: Fite Stain for Nocardia & Leprosy.)

URANYL NITRATE 1% Aqueous Solution
(use: Steiner Silver Impregnation Methods.)

WATER, DEIONIZED ACS REAGENT, CLSI Type 1

WEIGERT IODINE
See IODINE,Weigert, cata #12092 in Reagents, Chemicals & Buffers Section.

WRIGHT STAIN BUFFER SOLUTION pH 6.8

XYLENE ACS

 
 
 
 
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Ship Ground Only

XYLENE Histology Grade

 
 
 
Ship Ground Only
Ship Ground Only

XYLENE SUBSTITUTE
An extremely safe, odorless alternative to xylene. Gentle on tissue and does not make it brittle. Excellent for derms and biopsy tissue. No tissue shrinkage or morphology changes. Enhances nuclear detail. Non-flammable with a flash point of 142°F. Use with S-Mount Mounting Medium for optimal results. No hazardous shipping fees! See Slide Brite in Safety Section.

XYLENE / PEANUT OIL 2:1
(use: Fite Stain for Leprosy & Nocardia.)